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KMID : 0363320040250020214
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Journal of Korean Oriental Internal Medicine
2004 Volume.25 No. 2 p.214 ~ p.223
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Effect of Mylabris phalerata on colorectaladenocarcinoma cells (SNU-C5 cell lines)
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Kim Hyeon-Il
Kim Jin-Sung
Yoon Sang-Hyub
Ryu Bong-Ha
Ryu Ki-Won
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Abstract
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The study examines the anti-cancer effects of the hot water extract of Mylabris phalerata(MP) using SNU-C5 cell lines. Microscopic analysis showed that 12 hours after MP treatment, the number of dead cells increased prominently. Significant cell death was observed 12, 24, and 48 hours after MP treatment through trypan blue exclusion testing. This suggests that MP is time-dependently cytotoxic. Mitotracker Red CMXRos staining and flowcytometry revealed that MP decreased mitochondrial membrane potentials. The absence of peaks on PI staining showed that DNA damage occurred in MP treated cells. Taken together, measurements suggest that MP has a strong anti-cancer effect on SNU-5 cell lines, and that this is likely to be due to the destruction of mitochondria and DNA damage.
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KEYWORD
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My labris phalerata, membrane potentials
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